|Cell Line Name：||EGFR A763_Y764insFQEA/BaF3|
|Stability:||16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Application:||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.|
|Freeze Medium:||90% FBS+10% DMSO|
|Complete Culture Medium:||RPMI-1640+10%FBS+2ug/ml puromycin|
EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. EGFR A763_Y764insFQEA results in the insertion of four amino acids in the C-helix region of the Egfr protein between amino acids 763 and 764 (PMID: 24353160). A763_Y764insFQEA confers a gain of function to Egfr, as indicated by increased kinase activity and is transforming in cell culture (PMID: 24353160, PMID: 29533785).
|III. Representative Data|
1. WB of EGFR A763_Y764insFQEA/BaF3
2. Sanger of EGFR A763_Y764insFQEA/BaF3
3. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of BaF3 EGFR A763_ Y764insFQEA (C21).