|Cell Line Name：||EGFR H773_V774insAH/BaF3|
|Stability:||16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Application:||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.|
|Freeze Medium:||90% FBS+10% DMSO|
|Complete Culture Medium:||RPMI-1640+10%FBS|
EGFR H773_V774insAH results in the insertion of two amino acids within the drug-binding pocket in the protein kinase domain of the Egfr protein between amino acids 773 and 774 (UniProt.org, PMID: 23371856). H773_V774insAH has not been biochemically characterized, but is predicted to result in a gain of function based on the effect of other Egfr exon 20 insertion mutations (PMID: 24353160).
|III. Representative Data|
1. WB of EGFR H773_V774insAH/BaF3