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科研细胞

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药靶细胞

药靶细胞

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NGS靶向捕获探针

NGS靶向捕获探针
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NFkB-Luc/Jurkat

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产品编号
.CBP74031
货号:
.CBP74031
品牌:
Cobioer
规格:
T-25 Flask
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.CBP74031
I. Description
NF-kB luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-kB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.
 
II. Introduction
Host Cell: Jurkat
Expressed gene: NF-κB Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): Nfkb, nf-kb reporter, nfkb report, nf-kb jurkat
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+800ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
III. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Peripheral blood
Disease: Acute T cell leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
IV. Representative Data

Figure 1. Induction of NFkB activity by PMA with lonomycin in Jukart-NFkB Luciferase Reporter Cell.

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