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SBE-Luc/HEK293

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.CBP74058

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.CBP74058

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Cobioer

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T-25 Flask

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.CBP74058
I. Background
The transforming growth factor beta (TGFβ) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGFβ signaling has been linked to cardiac disease, cancer, Alzheimer’s and other human diseases. TGFβ proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocates to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGFβ/SMAD responsive genes.

II. Description
The SBE Reporter – HEK293 Cell Line is designed for monitoring the activity of the TGF /SMAD signaling pathway. The transforming growth factor beta (TGF) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGF signaling has been linked to cardiac disease, cancer, Alzheimer’s and other human diseases. TGF proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocates to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGF / SMAD responsive genes.

III. Introduction
Host Cell:	HEK293
Expressed gene:	SBE-Luciferase
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	SBE reporter, TGFβ reporter, TGFbeta reporter, SMAD reporter
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	DMEM+10%FBS+100ug/ml hygromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Homo sapiens, human
Tissue:	Embryonic kidney
Disease:	Normal
Morphology:	Epitheloid cell
Growth Properties:	Adherent

Ⅴ. Representative Data


Figure . Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/SBE Luciferase Reporter cells were stimulated by TGF, the S/B was 138.3-fold.