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科研细胞
药靶细胞
标准品
NGS靶向捕获探针
41BB/NFkB-Luc/HEK293
CBP74045 | |
I. Background | |
Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients. |
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II. Description | |
Recombinant HEK293 cell constitutively expressing full length human CD137 (Genbank #NM_003811). Surface expression is confirmed by flow cytometry. |
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III. Introduction | |
Host Cell: | HEK293 |
Expressed gene: |
4-1BB |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | 4-1BB, TNFRS9, Mitogen-Activated Protein Kinase 14, Signal Transducer And Activator Of Transcription 3, STAT3, MAPK14 |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: |
MEM+10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
IV. Description of Host Cell Line | |
Organism: | Human |
Tissue: | Embryonic kidney |
Disease: | Normal |
Morphology: | Epitheloid |
Growth Properties: | Adherent |
Ⅴ. Representative Data | |
Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of 4-1BB. |
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Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). 4-1BB/NF-κB Reporter-HEK293 cells were stimulated by 4-1BBL, the S/B was 14.1-fold. |

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