|B-Cell Maturation Antigen (BCMA), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17) or CD269, is a type I membrane protein encoded by the TNFRSF17 gene. TNFRSF17 is a cell surface receptor of the TNF receptor superfamily, that recognizes its ligands including BAFF (B-cell activating factor) and APRIL (a proliferation inducing ligand). BCMA is preferentially expressed in mature B lymphocytes and also on Multiple Myeloma (MM) cells. In vitro, the activation of BCMA by its ligand promotes the differentiation and proliferation of B cells. A vast array of intracellular activity is involved in BCMA-induced signal transduction, including activation of the NF-κB signaling pathway. The bioactivity of BAFF and APRIL as soluble homotrimers distinguishes them from other TNFSF ligands such as TRAIL, FasL, and CD40L, which are only active as membrane-bound molecules.|
The BCMA / NF-κB - HEK293 recombinant cell line has been transfected with full-length human BCMA cDNA (Genbank #NM_001192) under control of a CMV promoter for high constitutive expression. The NF-κB–luciferase reporter is also stably integrated into the genome.
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Synonym(s):||tumor necrosis factor receptor superfamily member 17, B-Cell Maturation Antigen, TNFRSF17, CD269|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +100ug/ml hygromycin+1ug/ml puromycin|
|Application(s):||Functional(Report Gene) Assay|
|IV. Description of Host Cell Line|
|Ⅴ. Representative Data|
Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of BCMA.
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). HEK293-BCMA-NFκB Luciferase Reporter cells were stimulated by rhBAFF, the EC50 was 34.6ng/ml.