Glucocorticoid-induced TNFR-related protein (GITR) is a member of the TNFR superfamily, expressed in a number of cells including T cells, natural killer (NK) cells and antigen-presenting cells (APC). GITRL, GITR’s natural ligand, is expressed mainly by APCs and GITR:GITRL interaction is important for activation of the immune system. Agonistic antibodies targeting GITR are actively being pursued as potential immuno-oncology therapies.
|This cell line expresses a surface human GITR (glucocorticoid-induced TNFR family related gene; TNFRSF18; CD357) and an NF-kB luciferase reporter construct that are stably integrated into the genome of Jurkat T-cells.|
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||RPMI-1640+10%FBS+400ug/ml hygromycin+1ug/ml puromycin|
|Application(s):||Functional(Report Gene) Assay|
|IV. Description of Host Cell Line|
|Disease:||Childhood T acute lymphoblastic leukemia|
|Ⅴ. Representative Data|
Figure 1. Recombinant Jurkat stably expressing human GITR.
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Jurkat/NF-κB Luciferase-GITR Reporter cells were stimulated by GITRL/CHO cells, the EC50 was 2634cells.
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Jurkat/NF-κB Luciferase-GITR Reporter cells were stimulated by hGITRL, the EC50 was 0.15ug/ml.