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ISRE-Luc/NFkB-SEAP/THP-1

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产品编号
CBP74097
货号:
CBP74097
品牌:
Cobioer
规格:
T-25 Flask
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CBP74097
I. Background

THP1 reporter cells are derived from THP-1, a human monocytic cell line that naturally expresses many pattern-recognition receptors, including Toll-like receptors.

 
II. Description

The NF-κB reporter (Luc)-THP-1 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

 
III. Introduction
Host Cell: THP-1
Expressed gene: ISRE
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, nfkb thp1, thp1, NF-kB, nfkb, nf-kb
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+10 µg/ml of blasticidin+ 100 μg/ml of Zeocin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Peripheral blood
Disease: Acute monocytic leukemia
Morphology: Monocyte
Growth Properties: Suspension
 
Ⅴ. Representative Data

 

 

 

 

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相关文件

  • Manual download:CBP74097 ISRE-Luc NFkB-SEAP THP-1.pdf

    大小:
    2023-06-08
  • ISRE-LucNFκB-SEAPTHP-1说明书.pdf

    大小:
    2024-04-02
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