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ISRE-Luc/NFkB-SEAP/THP-1

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CBP74097

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CBP74097

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Cobioer

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T-25 Flask

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CBP74097
I. Background
THP1 reporter cells are derived from THP-1, a human monocytic cell line that naturally expresses many pattern-recognition receptors, including Toll-like receptors.

II. Description
The NF-κB reporter (Luc)-THP-1 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

III. Introduction
Host Cell:	THP-1
Expressed gene:	ISRE
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, nfkb thp1, thp1, NF-kB, nfkb, nf-kb
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	RPMI-1640+10%FBS+10 µg/ml of blasticidin+ 100 μg/ml of Zeocin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Homo sapiens, human
Tissue:	Peripheral blood
Disease:	Acute monocytic leukemia
Morphology:	Monocyte
Growth Properties:	Suspension

Ⅴ. Representative Data
Figure 1. Dose Response of 2'3-cGAMP in THP-1 ISRE-Luc/NFkB-SEAP Cells , the EC50 was 13.5μM.