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LAG3/NFAT-Luc/Jurkat

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产品编号
CBP74060
货号:
CBP74060
品牌:
Cobioer
规格:
T-25 Flask
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CBP74060
I. Background

Lymphocyte-activation gene 3 (LAG3, CD223) is a cell surface protein that belongs to immunoglobulin (Ig) superfamily. LAG3 is expressed on activated T cells, natural killer cells, B cells, and plasmacytoid dendritic cells.

 
II. Description
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human LAG3 (lymphocyte-activation gene 3, CD223, GenBank Accession # NM_002286).
 
III. Introduction
Host Cell: Jurkat
Expressed gene: NFAT-LAG3-Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): LAG3, LAG-3, CD223, Cell Line, Jurkat
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+1ug/ml puromycin+400ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
IV. Description of Host Cell Line
Organism: Human
Tissue: Peripheral blood
Disease: Childhood T acute lymphoblastic leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
Ⅴ. Representative Data

Figure 1. Recombinant Jurkat stably expressing human LAG3.

 

Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). Dose Response of Anti-LAG3 Neutralizing Antibody in LAG3/NFAT Reporter-Jurkat cells.

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相关文件

  • Manual download:CBP74060 LAG3 NFAT-Luc Jurkat.pdf

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    2023-06-08
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