科研细胞

科研细胞



药靶细胞

药靶细胞



标准品

标准品



NGS靶向捕获探针

NGS靶向捕获探针



浏览量:

mouse ADCC Bioassay Effector Cell Line

所属分类

返回列表



零售价

0.0

元

市场价

0.0

元

浏览量:

产品编号

CBP74076

货号:

CBP74076

品牌:

Cobioer

规格:

T-25 Flask

物种:

组织:

数量

库存:

产品描述

参数

产品数据库

CBP74076
I. Background
Antibody-dependent cell-mediated cytotoxicity (ADCC) is an immune defense mechanism involving an effector cell lysing a target cell on which antibodies have bound to specific antigens on the target cell membrane. The typical ADCC involves activation of natural killer (NK) cells by antibodies. NK cells express Fc receptors, mostly CD16 or FcγRIIIa (CD16a), on its cell surface. These Fc receptors recognize and bind to the Fc portion of an antibody, such as IgG, which has bound to the surface of a pathogen-infected target cell. Once the Fc receptor binds to the Fc region of IgG, the Natural Killer cell releases cytokines such as IFN-γ and cytotoxic molecules that attack the pathogen-infected target cell.

II. Description
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of Mouse FcγRIV.

III. Introduction
Host Cell:	Jurkat
Expressed gene:	NFAT
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Homo sapiens, human
Tissue:	Peripheral blood
Disease:	Acute T cell leukemia
Morphology:	Lymphoblast
Growth Properties:	Suspension

Ⅴ. Representative Data

Figure 2. Dose response of mouse Anti-CD20 lgG2a in Mouse ADCC Bioassay Effector Cell (C3) .