Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40, is a member of the TNFR-superfamily of receptors. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation.
|Recombinant Jurkat cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327)|
|Stability:||32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Synonym(s):||OX40, OX40 cell Line, CD134 Cell Line, ox40 nfkb|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||RPMI-1640+10%FBS+400ug/ml hygromycin+1ug/ml puromycin|
|Application(s):||Functional(Report Gene) Assay|
|IV. Description of Host Cell Line|
|Disease:||Childhood T acute lymphoblastic leukemia|
|Ⅴ. Representative Data|
Figure 1. Recombinant Jurkat stably expressing human OX40.
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Jurkat-OX40-NFkB-Luciferase Reporter cells were stimulated by OX40L,the S/B was 5.8-fold.