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科研细胞
药靶细胞
标准品
NGS靶向捕获探针
OX40/NFkB-Luc/Jurkat
CBP74061 | |
I. Background | |
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40, is a member of the TNFR-superfamily of receptors. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation. |
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II. Description | |
Recombinant Jurkat cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327) | |
III. Introduction | |
Host Cell: | Jurkat |
Expressed gene: |
OX40-NFkB-Luciferase |
Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | OX40, OX40 cell Line, CD134 Cell Line, ox40 nfkb |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | RPMI-1640+10%FBS+400ug/ml hygromycin+1ug/ml puromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
IV. Description of Host Cell Line | |
Organism: | Human |
Tissue: | Peripheral blood |
Disease: | Childhood T acute lymphoblastic leukemia |
Morphology: | Lymphoblast |
Growth Properties: | Suspension |
Ⅴ. Representative Data | |
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Figure 1. Recombinant Jurkat stably expressing human OX40. |
Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat-OX40-NFkB-Luciferase Reporter cells were stimulated by OX40L,the S/B was 5.8-fold. |

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