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OX40/NFkB-Luc/Jurkat

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CBP74061

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CBP74061

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Cobioer

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T-25 Flask

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CBP74061
I. Background
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40, is a member of the TNFR-superfamily of receptors. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation.

II. Description
Recombinant Jurkat cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327)

III. Introduction
Host Cell:	Jurkat
Expressed gene:	OX40-NFkB-Luciferase
Stability:	32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	OX40, OX40 cell Line, CD134 Cell Line, ox40 nfkb
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	RPMI-1640+10%FBS+400ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Human
Tissue:	Peripheral blood
Disease:	Childhood T acute lymphoblastic leukemia
Morphology:	Lymphoblast
Growth Properties:	Suspension

Ⅴ. Representative Data

Figure 1. Recombinant Jurkat stably expressing human OX40.

Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Jurkat-OX40-NFkB-Luciferase Reporter cells were stimulated by OX40L,the S/B was 5.8-fold.