科研细胞

科研细胞



药靶细胞

药靶细胞



标准品

标准品



NGS靶向捕获探针

NGS靶向捕获探针



浏览量:

PDL1/TCR Activator/CHO

所属分类

返回列表



零售价

0.0

元

市场价

0.0

元

浏览量:

产品编号

CBP74066

货号:

CBP74066

品牌:

Cobioer

规格:

T-25 Flask

物种:

组织:

数量

库存:

产品描述

参数

产品数据库

CBP74066
I. Background
The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. PD-1 ligands are found on most cancers, and the PD-1:PD-L1/2 interaction inhibits T-cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

II. Description
Recombinant CHO-K1 cells constitutively expressing human PD-L1 (Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7- H1), GenBank accession # NM_014143) an engineered T cell receptor (TCR) activator.

III. Introduction
Host Cell:	CHO-K1
Expressed gene:	PDL1、TCR Activator
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	F12K+10%FBS+2ug/ml puromycin+500ug/ml hygromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Cricetulus griseus, hamster, Chinese
Tissue:	Ovary
Disease:	Hamster Chinese ovary
Morphology:	Epitheloid cell
Growth Properties:	Adherent

Ⅴ. Representative Data
Figure 1.Recombinant CHO cells constitutively expressing human PD-L1 and an engineered T-cell receptor (TCR) activator.
Figure 2.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose response curve of anti-PD-1 antibody on PD-1/PD-L1 Blockade Bioassay Model.
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer).Dose response curve of anti-PD-L1 antibody on PD-1/PD-L1 Blockade Bioassay Model.