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PDL2/TCR Activator/CHO

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CBP74065

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CBP74065

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Cobioer

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T-25 Flask

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CBP74065
I. Background
The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. PD-1 ligands are found on most cancers, and the PD-1:PD-L1/2 interaction inhibits T-cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

II. Description
Recombinant CHO-K1 cells constitutively expressing human PD-L2 (Programmed Cell Death 1 Ligand 2 or CD273, GenBank accession #NM_025239) and an engineered T-cell receptor (TCR) activator.

III. Introduction
Host Cell:	CHO-K1
Expressed gene:	PDL2
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	F12K+10%FBS+2ug/ml puromycin+500ug/ml hygromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Cricetulus griseus, hamster, Chinese
Tissue:	Ovary
Disease:	Hamster Chinese ovary
Morphology:	Epitheloid cell
Growth Properties:	Adherent

Ⅴ. Representative Data
Figure 1.Recombinant CHO-K1 cells constitutively expressing human PD-L2 (Programmed Cell Death 1 Ligand 2 or CD273, GenBank accession #NM_025239) and an engineered T-cell receptor (TCR) activator.

Figure 2. Dose responese curve of anti-PD-1 neutralizing antibody in PD-1/NFAT-Reporter/Jurkat cells with PD-L2/TCR activator/CHO.The bioassay consists of two genetically engineered cell lines, Jurkat/PD-1/NFAT-Luc Cells and CHO/TCR activator/PD-L2 Cells. When co-cultured, the PD-1/PD-L2 interaction inhibits TCR-mediated luminescence. When the PD-1/PD-L2 interaction is disrupted, TCR activation induced luminescence (via activation of NFAT pathway) that can be detected and quantified using Bio-Glo Reagents