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科研细胞
药靶细胞
标准品
NGS靶向捕获探针
PDL2/TCR Activator/CHO
CBP74065 | |
I. Background | |
The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. PD-1 ligands are found on most cancers, and the PD-1:PD-L1/2 interaction inhibits T-cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes. | |
II. Description | |
Recombinant CHO-K1 cells constitutively expressing human PD-L2 (Programmed Cell Death 1 Ligand 2 or CD273, GenBank accession #NM_025239) and an engineered T-cell receptor (TCR) activator. | |
III. Introduction | |
Host Cell: | CHO-K1 |
Expressed gene: | PDL2 |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | F12K+10%FBS+2ug/ml puromycin+500ug/ml hygromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
IV. Description of Host Cell Line | |
Organism: | Cricetulus griseus, hamster, Chinese |
Tissue: | Ovary |
Disease: | Hamster Chinese ovary |
Morphology: | Epitheloid cell |
Growth Properties: | Adherent |
Ⅴ. Representative Data | |
Figure 1.Recombinant CHO-K1 cells constitutively expressing human PD-L2 (Programmed Cell Death 1 Ligand 2 or CD273, GenBank accession #NM_025239) and an engineered T-cell receptor (TCR) activator. |
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Figure 2. Dose responese curve of anti-PD-1 neutralizing antibody in PD-1/NFAT-Reporter/Jurkat cells with PD-L2/TCR activator/CHO.The bioassay consists of two genetically engineered cell lines, Jurkat/PD-1/NFAT-Luc Cells and CHO/TCR activator/PD-L2 Cells. When co-cultured, the PD-1/PD-L2 interaction inhibits TCR-mediated luminescence. When the PD-1/PD-L2 interaction is disrupted, TCR activation induced luminescence (via activation of NFAT pathway) that can be detected and quantified using Bio-Glo Reagents |

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