|TIGIT is a co-inhibitory receptor that is highly expressed in Natural Killer (NK) cells, activated CD4+, CD8+ and regulatory T cells. Interaction with the poliovirus receptor (PVR; CD155) on antigen presenting cells, such as dendritic cells, recruits Src homology (SH) domain-containing protein tyrosine phosphatase SHP1 and SHP2 or the inositol phosphatase SHIP1 and SHIP2 to the TIGIT ITIM domain. This increases IL-10 release and suppresses NF-kB and NFAT T cell receptor (TCR) signaling, which blocks T cell proliferation and cytokine production. It serves as a competitive inhibitor of CD226, a co-stimulatory receptor for CD155. TIGIT targeting antibodies that block this T cell-intrinsic inhibitory effects have shown enhanced anti-tumor and anti-viral functions in preclinical studies.|
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human TIGIT (V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, and VSTM3, GenBank Accession No. NM_173799).
|Stability:||32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)|
|Synonym(s):||T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, VSTM3|
|Freeze Medium:||90% FBS+10% DMSO|
|Culture Medium:||RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin|
|Application(s):||Functional(Report Gene) Assay|
|IV. Description of Host Cell Line|
|Organism:||Homo sapiens, human|
|Disease:||Acute T cell leukemia|
|Ⅴ. Representative Data|
Figure 1.Recombinant Jurkat cell line expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of TIGIT.
Figure 2. Dose response of anti-TIGIT neutralizing antibody in TIGIT/NFAT-Reporter Jurkat cell line (C26) with CD155/TCR activator-CHO, the EC50 was 1.24μg/ml.
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