南京科佰生物科技有限公司官方网站欢迎您的光临!

免费咨询热线: 4008-750-250

搜索
确认
取消
/
/
/
/
hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Reporter Cell

科研细胞

科研细胞

药靶细胞

药靶细胞

标准品

标准品

NGS靶向捕获探针

NGS靶向捕获探针
浏览量:
1000

hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Reporter Cell

所属分类
返回列表
1
零售价
0.0
市场价
0.0
浏览量:
1000
产品编号
CBP74192
货号:
CBP74192
品牌:
Cobioer
规格:
T-25 Flask
物种:
组织:
数量
-
+
库存:
0
产品描述
参数
产品数据库
CBP74192
I. Background

The particles contain a firefly luciferase gene driven by the human IL-2 promoter. After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity.

IL-15 has been shown to be produced by human fetal astrocytes and microglial cells in response to IL-1beta, IFN-gamma, or TNF- alpha and may thus play a role in T-cell mediated immune responses in the human central nervous system. IL-15 stimulates proliferation of the established T-cell line CTLL-2, CD8(+) memory T- cells require IL-15 for proliferation. IL-15 is also able to induce generation of cytolytic cells and LAK cells activity in vitro. IL-15 appears to function as a specific maturation factor for NK-cells and can mimic the bone marrow microenvironment in vitro, which is required for NK- cells to acquire lytic functions. IL-15 induces proliferation of mast cells in the absence of functional IL-2 receptor components and utilizes a receptor of 60-65 kDa that is distinct from known components of T-cell IL-15 receptors. The IL-15 receptor on mast cells recruits JAK2 and STAT5, instead of JAK1, JAK3, and STAT3 and STAT5 that are activated in T-cells. The alpha subunit of the IL-15 receptor (IL-15RA) is structurally related to the alpha subunit of the IL-2 receptor and confers high affinity binding of IL-15 to its receptor. IL-15 binds to the beta and gamma chains of the IL2 receptor, which are known to be required for ligand internalization and signal transduction.

 
II. Introduction
Expressed gene: IL2R、IL15R
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+1ug/ml puromycin+800ug/ml hygromycin+10ug/ml blasticidin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
III. Representative Data

Figure 1. Recombinant hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Reporter Cell constitutively expressing IL2Rα.

Figure 2. Recombinant hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Reporter Cell constitutively expressing IL2Rβ.

Figure 3. Recombinant hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Reporter Cell constitutively expressing IL2Rγ.

Figure 4. Dose Response of Recombinant Human IL2/IL15 in hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132)Reporter Cells (C26).

 
未找到相应参数组,请于后台属性模板中添加

相关文件

  • hIL2IL15 Dual Effector(hCD25hCD122hCD132) Reporter Cell CBP74192说明书.pdf

    大小:
    2024-04-02
上一页
1

相关产品推荐

暂时没有内容信息显示
请先在网站后台添加数据记录。

联系我们

地址:南京市秦淮区普天路1号/实验室地址:上海市浦东新区广丹路222号 

邮箱: sales@cobioer.com (销售部) tech@cobioer.com (技术部)

 COBIOER

扫一扫,关注我们公众号

Copyright ©2021 www.cobioer.com All Rights Reserved. 南京科佰生物科技有限公司版权所有 苏ICP备13038923号