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PD1/NFAT-Luc/Jurkat

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CBP74018

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CBP74018

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Cobioer

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T-25 Flask

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CBP74018
I. Background
This PD-1 Cell Line is used to measure the binding of PD-1 to PD-L1/PD-L2. Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T cells, binds to its ligands, PD-L1 and PD-L2, to negatively regulate immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

II. Description
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_005018).

III. Introduction
Host Cell:	Jurkat
Expressed gene:	PD1、NFAT-Luciferase
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L, PD1/NFAT, PD-1 NFAT, PD-1/NFAT
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Homo sapiens, human
Tissue:	Peripheral blood
Disease:	Acute T cell leukemia
Morphology:	Lymphoblast
Growth Properties:	Suspension

Ⅴ. Representative Data

Figure 1.Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_005018).
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer).Dose response curve of anti-PD-1 antibody on PD-1/PD-L1 Blockade Bioassay Model.

Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose response curve of anti-PD-L1 antibody on PD-1/PD-L1 Blockade Bioassay Model.
Figure 4. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of PD-1 Agonist Antibody in PD-1/NFAT-Luc/Jurkat Cells With FCγR aAPC Cell.