HPV34/HEK293
CBPK0277
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| I. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | HPV34 (Full Length) |
| Genebank: | X74476.1 |
| HPV cross testing: | Not Found By dPCR |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: |
DMEM+10%FBS+2 μg/ml Puromycin
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| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| III. Representative Data | |
 Figure 1. Digital PCR data: HPV34 specific probe, HPV34 HEK293 is positive for HPV34, copy number data: 2394 copies/ng, reference gene is RPP30.  Figure 2. Sanger sequening of HPV34 L1 gene.Match rate 100%. |
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| Reference sequence： | |
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ATGGGTCCGATTTTATATTGCATCCTAGCTTGTATGTCATTCCACGCAAACGTAAACGTTTGTCATATTTTTTTGCAGATGTGGCGACCTACT
GAGGCAAAGGTATACCTGCCCCCTGTGTCTGTATCTAAAGTTGTAAGCACTGAAGAGTATGTAACACGCACAAATATATATTATTATGCAGG TAGTACACGCTTGCTGGCAGTAGGACATCCCTATTATCCTATAAAGGATACTAATGGGAAACGTAAGATTGCTGTACCTAAAGTTTCAGGTT TGCAATACAGGGTATTTAGAATACGTTTGCCAGATCCCAATAAATTTGGGTTCCCAGATGCATCTTTTTATAATCCTGACAAGGAGCGGTTG GTTTGGGCCTGTGCAGGCGTTGAGGTTGGACGCGGACAGCCATTAGGTATAGGTACTAGTGGTAATCCTTTTATGAATAAACTTGAGGATA CTGAAAATGCTGCAAAATATATTGGTGGAAACATAGCAGATAGTAGGGAGTGTATGTCAGTAGATTATAAACAAACACAGTTATGTATTGTA GGCTGTAAGCCTCCCTTAGGGGAACATTGGGGTACAGGCACACCATGTGGTACCCAAAATGCTGGGGAATGTCCTCCGCTTGAATTAAAG AACACCACTATACAGGATGGTGATATGATTGATGTGGGCTTTGGTGCCATGGATTTTAAGGCCTTACAGGCAAATAAAAGTGATGTGCCCAT TGATATTTCCAACACTATATGTAAATATCCAGATTATCTAGGCATGGCCGCAGATCCCTATGGCGATTCTATGTGGTTTTATATTCGTAGGGAA CAAATGTTTGTTAGACACCTATTTAACAGGGCAGGTACTGTAGGCGATGCTATTCCAGATGACTTAATGATTAAGGGTACAGGCAATACTGC ATCGCCATCCAGTTGTGTGTTTTATCCTACTCCTAGTGGGTCTATGGTATCCTCAGATGCACAAATTTTTAATAAGCCTTATTGGTTGCAAAAG GCCCAGGGACAAAACAATGGCATTTGCTGGCATAATCAACTGTTTTTAACTGTTGTAGATACTACTAGAAGCACAAACTTTTCAGTTTGTGT AGGTACACAATCCACAAGTACAACTGCACCATATGCAAACAGTAATTTTAAGGAATACCTCAGACATGCAGAAGAGTATGACCTGCAGTTT GTGTTTCAGTTATGCAAAATCAATTTAACTACAGATGTAATGACATATATACATTCTATGAGTTCTAGTATATTGGAACAGTGGAATTTTGGTC TTACACCACCGCCTTCAGGTACTTTAGAAGAAACATATAGATATGTTACTTCACAGGCCATTACATGTCAGCGTCCGCAACCTCCTAAGGAA ACAGAGGACCCATATGGTAAAATGACATTTTGGGAGGTAGACCTTAAAGAAAAATTTTCTGCAGAATTAGATCAGTTTGCCTTAGGTAGAA AGTTTTTATTACAACTAGGTATGCGTGCCCGTCCTAGGTTACAGGCCTCTAAACGGTCTGCACCTTCATCATCTAGTACAGCACCTAAGAAA AAACGTGCAAAACGTATTTAA |
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