hNectin4/MC-38
CBPG0035
| I. Background | |
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Nectin 细胞粘附分子 4 (Nectin4)，是一种 I 型跨膜糖蛋白，属于 Ig 超家族蛋白中的Nectin 家族。除 Nectin-4 外，还有其他三种 Nectin：Nectin-1、Nectin-2 和 Nectin-3。所有Nectin 都具有相同的结构，由三个细胞外免疫球蛋白样结构域（两个免疫球蛋白样 (Ig-like)C2 型结构域和一个 Ig-like V 型结构域）、一个跨膜区和一个胞内结构域组成。可溶形式的Nectin-4 蛋白是由金属蛋白酶 ADAM17/TACE 在细胞表面进行蛋白水解裂解而产生的。 Nectin4 在多种肿瘤细胞中过表达，也能促进肿瘤细胞的生长和增殖。高表达 Nectin4的实体肿瘤包括膀胱癌、胰腺癌、三阴性乳腺癌、非小细胞肺癌、胃癌、食道癌、卵巢癌等多种癌症。 |
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| II. Introduction | |
| Host Cell: | MC-38 |
| Expressed gene: | hNectin4 |
| Stability: | 32 passages |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+5μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mouse |
| Tissue: | Colon |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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Figure 1. Recombinant hNectin4/MC38 stably expressing hNectin4. |
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| V. In VIVO Data | |
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Index |
Content |
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Animal |
C57BL/6J, 7weeks, female |
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Implanted Position |
Subcutaneous (right lower flank region ) |
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Injected Cell Numbers |
1×10^6/0.1 ml |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hNectin4/MC38 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3. hNectin4/MC38 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
