OVA/B16-F10
CBPG0012
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产品描述
产品数据库
| I. Background | |
| OVA 基因（Ovalbumin）是鸡卵清蛋白编码基因，分子量约 43kDa，含 12269 个氨基酸，为免疫学与肿瘤研究的核心工具。其表位如 SIINFEKL 可激活 CD8+ T 细胞，用于构建稳转细胞系评估 CAR-T/TCR-T 疗效及疫苗免疫应答。OVA 四聚体技术可精准检测抗原特异性 T 细胞，在肿瘤免疫监测、表观遗传调控（如 DNA 甲基化）及胚胎发育研究中发挥关键作用，是精准免疫医学的重要实验平台。 | |
| II. Introduction | |
| Host Cell: | B16-F10 |
| Expressed gene: | OVA |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mus musculus, mouse |
| Tissue: | skin |
| Morphology: | mixture of spindle-shaped and epithelial-like cells |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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Figure 1. WB of OVA/B16-F10. |
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| V. In VIVO Data | |
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Index |
Content |
|
Animal |
C57BL/6J, 7weeks, male |
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Implanted Position |
Subcutaneous (right lower flank region ) |
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Injected Cell Numbers |
2×10^5/0.1ml |
|
Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. OVA/B16-F10 Mean Body Weight ± SEM.
2. Tumor Volume
Figure 3. OVA/B16-F10 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
