hITGA6&hITGB4/MC-38
CBPG0244
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| I. Background | |
| ITGA6 与 ITGB4 是整合素家族的关键成员，分别编码α6 和β4 亚基，通过非共价结合 形成α6β4 整合素复合体，在细胞粘附、迁移及信号传导中发挥核心作用。ITGA6 位于 2q31.1，编码整合素α6 亚基，可与β1 或β4 结合，参与细胞与层粘连蛋白的相互作用，调控 细胞增殖、组织再生及肿瘤侵袭，其异常表达与交界性大疱性表皮松解症、卵巢癌、结直 肠癌及肾病综合征相关，是潜在治疗靶点。ITGB4 位于 17q25，编码整合素β4 亚基，与α6 结合后形成层粘连蛋白受体，通过 FAK/Src、EGFR-AKT 等信号通路影响细胞极性、肿瘤 进展及耐药机制，其突变直接导致伴幽门闭锁的大疱性表皮松解症，并在胰腺癌、非小细 胞肺癌中高表达，与患者预后密切相关。两者形成的α6β4 整合素在肿瘤生物学中尤为关键， 如在卵巢癌中表达显著上调且呈正相关，可作为诊断标志物；在胰腺癌中则通过调控 AREG、EREG 和 MMP1 等基因促进侵袭转移。 | |
| II. Introduction | |
| Host Cell: | MC-38 |
| Expressed gene: | ITGA6&ITGB4 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin+5 μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mus musculus, mouse |
| Tissue: | Colon |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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| V. In VIVO Data | |
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Index |
Content |
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Animal |
C57BL/6J, 6weeks, female |
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Implanted Position |
Subcutaneous (right lower flank region) |
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Injected Cell Numbers |
1×10^6/0.1ml |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hITGA6&hITGB4/MC38 Mean Body Weight ± SEM.
2. Tumor Volume
Figure 3. hITGA6&hITGB4/MC38 Mean Tumor Volume ± SEM.
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
