hPDL1/MC-38

CBPG0001

询价

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产品描述

产品数据库

I. Background

Programmed Cell Death Protein 1（PD-1），一种在激活的 T 细胞上表达的受体，与其配体 PD-L1 和 PD-L2 结合，负向调节免疫反应。 PD-1 配体存在于大多数癌症中，PD- 1:PD-L1/2 相互作用会抑制 T 细胞活性，并使癌细胞逃避免疫监视。 PD1/PDL1 信号转导通路是肿瘤免疫抑制的重要组成部分，可以抑制 T 淋巴细胞的兴奋，增强肿瘤细胞的免疫耐受，从而实现肿瘤免疫逃逸。PD1 与 PDL1 结合可以减弱 T 细胞介导的免疫监视，导致免疫反应缺失，甚至导致 T 细胞凋亡。PD1/PDL1 抑制剂可解除抗肿瘤 T 细胞的免疫抑制，从而导致 T 细胞增殖并渗透到肿瘤微环境中并诱导抗肿瘤反应。PD-1:PD-L1/2 通路还参与调节自身免疫反应，使这些蛋白质成为多种癌症以及多发性硬化症、关节炎、狼疮和 I 型糖尿病的有希望的治疗靶点。

II. Introduction

Host Cell:

MC38

Expressed gene:

hPD-L1

Stability:

32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)

Freeze Medium:

90% FBS+10% DMSO

Culture Medium:

DMEM+10%FBS+10 μg/ml blasticidin

Mycoplasma Testing:

Negative

Storage:

Liquid nitrogen

III. Description of Host Cell Line

Organism:

Mouse

Tissue:

Colon

Morphology:

Epitheloid cell

Growth Properties:

Adherent

IV. Representative Data

Figure 1. Recombinant hPDL1/MC38 stably expressing PDL1.

V. In VIVO Data

Index	Content
Animal	C57BL/6J, 7weeks, female
Implanted Position	Subcutaneous (right lower flank region )
Injected Cell Numbers	1×10^6/0.1ml
Matrix gel	Without matrix gel

1. Weight Change

Figure 2. hPDL1/MC38 Mean Body Weight ± SEM.

2. Tumor Size

Figure 3. hPDL1/MC38 Mean Tumor Volume ± SEM.

NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).