647-V(人恶性2级膀胱癌细胞)
CBP60831
                       
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                | I. General information | |
| Synonyms: | 647-V | 
| Background: | established from a male patient with a primary urothelial bladder carcinoma (malignant, grade 2) | 
| Species: | human (Homo sapiens) | 
| Tissue: | bladder | 
| Disease: | urothelial bladder carcinoma | 
| Gender: | N/A | 
| Morphology: | adherent epithelial-like cells growing in monolayers | 
| Growth Mode: | N/A | 
| Doubling Time: | ca. 30 hours | 
| DNA Profile: | N/A Our Cell Line Authentication Service  | 
		
| Culture Medium: | 
				 DMEM+10%FBS 647-V完全培养基,# CBP60831M  | 
		
| Cryopreservation medium: | 90%FBS+10%DMSO | 
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays | 
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - | 
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile | 
| Species: | confirmed as human with IEF of AST, NP | 
| Cytogenetics: | human flat-moded hyperdiploid/hypotriploid karyotype with 12% polyploidy - 52-60<3n>XX, -X/Y, -2, -4, -6, -9, -10, -12, -12, -14, -15, -16, -17, -18, +5mar, del(1)(p32), del(2)(q23)/add(2)(q2?), add(3)(q27), add(3)(q1?), i(5p), i(6p), i(7p)x2, add(8)(q24), del(11)(q22q24), del(13)(q?11q?14), der(19)t(12;19)(q13;q13), add(21)(p11) - i(5p) associated with bladder cancer | 
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - | 
| Comments: | For more information, please contact us (4008-750-250). |