HHLA2(B7H7) aAPC Cell
CBP74194
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| I. Background | |
| HHLA2（也称为 B7H5、B7H7 或 B7y）是 B7 家族的一员，它是 I 型跨膜蛋白，由具 有串联 IgV1-IgC-IgV2 结构域的细胞外部分、跨膜区域和细胞质尾部组成。HHLA2 低表达 于胎盘、肠道上皮细胞等，高表达于多种实体瘤（如肺癌、乳腺癌、结直肠癌、肾癌）及 炎症微环境。HHLA2 具有双向免疫调节功能：结合抑制性受体 KIR3DL3（NK 细胞）和未 知 T 细胞受体，传递抑制信号，减弱抗肿瘤免疫应答；通过受体 TMIGD2 激活 PI3K-AKT 通路，促进 T 细胞存活及记忆形成。 | |
| II. Description | |
HHLA2(B7H7) aAPC Cell 作 为 KIR3DL3 Effector Reporter Cell 或 TMIGD2 Effector Reporter Cell 的 靶 细 胞 ， 很好的模拟了体内 KIR3DL3/ HHLA2(B7H7) 或TMIGD2/HHLA2(B7H7)的信号转导过程，原理见下图所示。  Figure 1. HHLA2(B7H7) aAPC Cell 细胞模型原理图 |
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| II. Introduction | |
| Expressed gene: | HHLA2(B7H7) |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+2ug/ml puromycin+500ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
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Figure 1.Recombinant HHLA2(B7H7) aAPC Cell stably expressing HHLA2(B7H7). |
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Figure 2. Dose Response of HHLA2 Blocking Abs in KIR3DL3 Effector Reporter Cells (C1)With HHLA2(B7H7) aAPC Cell(C1). |
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Figure 3. Dose Response of HHLA2 Blocking Abs in TMIGD2 Effector Reporter Cells (C1) With HHLA2(B7H7) aAPC Cell. |
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