HVEM/NFκB-Luc/Jurkat
CBP74013
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| I. Background | |
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疱疹病毒进入介质(HVEM),也称为肿瘤坏死因子受体超家族成员 14 (TNFRSF14),是 TNF 受体(肿瘤坏死因子)超家族的人细胞表面受体。HVEM 在多种 免疫细胞中表达,包括 T 细胞、B 细胞、自然杀伤细胞、树突状细胞和内皮细胞。它还通 过在黑色素瘤细胞上的频繁表达参与肿瘤的免疫逃避。 |
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| II. Description | |
HVEM NFκB-Luc Jurkat 报告基因药靶模型很好的模拟了体内 HVEM 的信号转导过程, 原理见下图所示。![]() Figure 1. HVEM NFκB-Luc Jurkat 细胞模型原理图 |
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| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: |
HVEM-NFκB-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | HVEM, TNFRSF14, CD270, HVEA, TR2, LIGHTR, HVEM Cell line, HVEM NFAT |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+800μg/ml hygromycin+1μg/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Peripheral blood |
| Disease: | Childhood T acute lymphoblastic leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
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Figure 2. Recombinant Jurkat stably expressing human HVEM. |
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