mouse ADCC Bioassay Effector Cell
CBP74076
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| I. Background | |
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抗体依赖的细胞介导的细胞毒性作用（ADCC，antibody-dependent cell-mediated cytotoxicity ）是指抗体的 Fab 段结合病毒感染的细胞或肿瘤细胞的抗原表位，其 Fc 段与杀 伤细胞（NK 细胞、巨噬细胞等）表面的 FcR 结合，介导杀伤细胞直接杀伤靶细胞，它是 抗肿瘤的治疗性抗体药物发生作用的一种作用重要机制。 |
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| II. Description | |
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Mouse ADCC Bioassay Effector Cell 报告基因药靶模型很好的模拟了体内 ADCC 的信号转导过程，原理见下图所示。
Figure 1. Mouse ADCC Bioassay Effector Cell 模型原理图 |
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| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | FcγRⅣ |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+800 μg/ml hygromycin+1 μg/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
Figure 2. Dose response of Anti-hCD20-mlgG2a in Mouse ADCC Bioassay Effector Cell (C3).
