hHER2/EMT6
CBPG0027
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| I. Background | |||
| HER2 基因属于人表皮生长因子受体家族之一（ErbB 家族），该家族由 EGFR、HER2、 HER3 和 HER4 四个高度同源的蛋白成员组成，控制着细胞生长、存活分化和迁移。与其 他三个成员不同的是，HER2 蛋白在本质上是一个孤儿受体，缺乏特异性配体，是唯一 一 个可以与家族其他成员结合形成二聚体结构的受体。HER2 蛋白在与细胞外配体结合后， 受体二聚形成异二聚体，随后自身磷酸化，进而激活细胞内信号通路，如丝裂原活化蛋白 激酶/ERK 通路、磷脂酰肌醇 3 激酶/Akt/mTOR 通路等，下游信号通路的激活可调节细胞增 殖、生存、以及肿瘤细胞的转移等。HER2 在正常情况下表达低或不表达，但在各种实体 瘤中经常过表达，HER2 与多种癌症相关，包括乳腺癌、胃癌、结肠癌、膀胱癌、卵巢癌、 子宫内膜癌、肺癌等。 | |||
| II. Introduction | |||
| Host Cell: | EMT6 | ||
| Expressed gene: | HER2 | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | RPMI-1640+10%FBS+2 μg/ml Puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mus musculus, mouse | ||
| Tissue: | breast | ||
| Morphology: | Epithelial | ||
| Growth Properties: | Adherent | ||
| IV. Representative Data | ||||||||||||||||||||||
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Figure 1. Recombinant hHER2/EMT6 stably expressing ERBB2. |
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| V. In VIVO Data Data of Experiment 1：
1. Weight Change  Figure 2. Overall effects of different treatments on mouse body weight changes. 2. Tumor Size  Figure 3. Changes in tumor volume over time in mice from different treatment groups. Data of Experiment 2：
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1. Weight Change
Figure 4. hHER2/EMT6 Mean Body Weight ± SEM.
2. Tumor Size
Figure 5. hHER2/EMT6 Mean Tumor Volume ± SEM .
NOTE：Data for Experiment 2 were provided by Cobioer (cell data) and Truway Bio (in vivo data model).
