hLRRC15/4T1
CBPG0123
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| I. Background | |||
| LRRC15(Leucine Rich Repeat Containing 15),富含 15 个亮氨酸的重复序列,是 LRRC 超家族的成员之一。LRRC15 同时能够正调控细胞的迁移,调控受体介导的病毒粒 子与宿主细胞的附着,且蛋白质定位于细胞质膜。LRRC15 主要是以蛋白-蛋白和蛋白-基质 相互作用方式,参与细胞-细胞和/或-细胞外基质的相互作用,参与炎性反应相关疾病,同 时在细胞粘附、信号转导、DNA 修复等过程中有重要作用。LRRC15 在正常组织中的低表 达,而在骨肉瘤、卵巢癌、乳腺癌、胰腺导管腺癌、促结缔组织增生型小圆细胞癌等癌症 中高表达。 | |||
| II. Introduction | |||
| Host Cell: | 4T1 | ||
| Expressed gene: | hLRRC15 | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | RPMI-1640+10%FBS+2 μg/ml puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mouse | ||
| Tissue: | Mammary gland | ||
| Morphology: | Epithelial | ||
| Growth Properties: | Adherent | ||
IV. Representative Data![]() |
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Figure 1. Recombinant hLRRC15/4T1 stably expressing hLRRC15. |
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