hEpCAM/MC-38

CBPG0151

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I. Background
EpCAM（Epithelial Cell Adhesion Molecule，上皮细胞粘附分子，CD326）是一种跨膜糖蛋白，在细胞的信号传导、粘附、细胞增殖分化、器官形成和维持中起着重要作用。 EpCAM 基因在人胚胎期除了胸腺组织以外的几乎所有的上皮组织中表达。在正常成人组织中，EpCAM 在胃肠道、生殖系统和呼吸道的各种组织中的简单、假复层和移行上皮细胞的基底外侧面表达。EpCAM 发生功能缺失性突变会导致先天性簇绒性肠病，在病理条件下，EpCAM 在起源于上皮细胞的癌症中特异性表达。此外，EpCAM 在肺癌、乳腺癌、宫颈癌和胰腺癌等多种癌症中高表达。

II. Introduction
Host Cell:	MC-38
Expressed gene:	hEpCAM
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	DMEM+10%FBS+5 μg/ml puromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen

III. Description of Host Cell Line
Organism:	Mus musculus, mouse
Tissue:	Colon
Morphology:	Epitheloid cell
Growth Properties:	Adherent

IV. Representative Data
Figure 1. Recombinant hEpCAM/MC-38 stably expressing hEpCAM.

V. In VIVO Data

Index	Content
Animal	C57BL/6J, 6weeks, female
Implanted Position	Subcutaneous (right lower flank region)
Injected Cell Numbers	1×10^6/0.1ml
Matrix gel	Without matrix gel

1. Weight Change

Figure 2. hEpCAM/MC38 Mean Body Weight ± SEM.

2. Tumor Volume

Figure 3. hEpCAM/MC38 Mean Tumor Volume ± SEM.

NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).