CCR8/CHO
CBP71364
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| I. Background | |
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趋化因子(C-C motif)受体 8 (CCR8)是一种主要表达于调节性 T 细胞(Treg)上的趋化因子 受体，属于 G 蛋白偶联受体。CCR8 的配体有 CCL1、CCL8、CCL16 和 CCL18。 |
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| II. Description | |
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cAMP 在 G 蛋白偶联受体(GPCR)信号转导中是一种关键的第二信使。当配体结合到 GPCR，构象发生变化，激活受体接着激活 G 蛋白，Gi 的激活导致腺苷酸环化酶调控 cAMP 的下调。CCR8 药靶模型很好的模拟了体内 CCR8 的信号转导过程，原理见下图所示。
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| III. Introduction | |
| Host Cell: |
CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5μg/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for CCR8 |
| Transducer: | Gi |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
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Figure 2. Recombinant CCR8/CHO constitutively expressing CCR8. |
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Figure 3. HTRF cAMP Assay with CCR8 CHO(C36). |
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