CALCRL&RAMP1&VPAC1 CRE-Luc HEK293
CBP71377
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| I. Background | |
| CALCRL（降钙素受体样受体）与RAMP1（受体活性修饰蛋白1）共同形成功能性降钙素基因相关肽（CGRP）受体，主要介导血管舒张、疼痛传递和神经源性炎症，是偏头痛等疾病的关键治疗靶点。VPAC1（血管活性肠肽受体1）是B类G蛋白偶联受体（GPCR），是血管活性肠肽（VIP）和垂体腺苷酸环化酶激活肽（PACAP）的高亲和力受体，主要激活Gs/cAMP通路，在免疫调节、神经保护及胃肠道稳态中发挥核心作用。两者虽同属B类GPCR家族，但配体、复合物组成及生理病理功能各不相同。 | |
| II. Description | |
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N/A
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III. Introduction |
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| Expressed gene: |
CALCRL、RAMP1、VPAC1 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin+ 5 μg/ml Blasticidin+200 μg/ml Hygromycin B+ 400 μg/ml G418 |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): | Functional assay for CALCRL&RAMP1&VPAC1 |
| Transducer: | Gs |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | kidney |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
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