ERBB Family Effector Reporter Cell
CBP74507
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产品数据库
| I. Background | |
| ERBB 家族（又称 HER家族）是一类在细胞生长、分化与存活中至关重要的受体酪氨酸激酶，其异常激活是驱动多种癌症（如肺癌、乳腺癌、胃癌）的关键因素。该家族包含 四名成员：EGFR/HER1、HER2、HER3 和 HER4。它们各自的特点为：EGFR 拥有多种配 体；HER2 无已知直接配体但信号能力最强；HER3 激酶活性缺失，必须与其他成员协作； HER4 结构最为复杂。其中，HER2 是最核心的信号放大器，而 HER3 是关键的信号伙伴， 两者的异源二聚体是驱动肿瘤生长和耐药的主要信号单位。 ERBB 家族通过配体诱导的二聚化机制启动其核心信号通路。当生长因子（配体）结合后，受体形成同源或异源二聚体（尤其是以 HER2 为中心的异源二聚体），引发胞内酪氨酸激酶区的相互磷酸化。这些磷酸化位点随后募集并激活下游两条主要信号通路： RAS/MAPK 通路（调控基因表达和细胞增殖）与 PI3K/AKT 通路（调控细胞存活和代谢）。这条从膜受体到细胞核的强大信号传导级联，在正常情况下精密调控生理过程，一 旦因受体过表达或突变而失控，便会持续驱动肿瘤的生长、侵袭和生存，这也正是针对 EGFR 和 HER2 等靶点的药物（如单克隆抗体、小分子抑制剂）能够有效治疗癌症的根本原理。 |
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| II. Description | |
ERBB Family Effector Reporter Cell 报告基因药靶模型很好的模拟了体内 ERBB 家族的信号转导过程，原理见下图所示。 Figure 1. ERBB Family Effector Reporter Cell 细胞模型原理图 |
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| III. Introduction | |
| Expressed gene: | EGFR&HER2&HER3&HER4 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640 +10%FBS + 100 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
 Figure 2. WB of ERBB Family Effector Reporter Cell.  Figure 3. Recombinant ERBB Family Effector Reporter Cell stably expressing EGFR & ERBB2 & ERBB3.  Figure 4. Dose Response of Ligands in ERBB Family Effector Reporter Cell (C16).  Figure 5. Inhibition of Human EGF induced Reporter Activity by ERBB Family Blocking Abs in ERBB Family Effector Reporter Cell(C16).  Figure 6. Inhibition of Human EGF induced Reporter Activity by ERBB Family Small Molecular Inhibitor in ERBB Family Effector Reporter Cell( C16).  Figure 7. Inhibition of Human NRG1-β1 induced Reporter Activity by ERBB Family Blocking Abs in ERBB Family Effector Reporter Cell(C16).  Figure 8. Inhibition of Human NRG1-β1 induced Reporter Activity by ERBB Family Small Molecular Inhibitor in ERBB Family Effector Reporter Cell (C16).  Figure 9. Inhibition of Human NRG1-β2 induced Reporter Activity by ERBB Family Blocking Abs in ERBB Family Effector Reporter Cell(C16).  Figure 10. Inhibition of Human NRG1-β2 induced Reporter Activity by ERBB Family Small Molecular Inhibitor in ERBB Family Effector Reporter Cell (C16). |
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